Composite

Part:BBa_I712029:Design

Designed by: Anja Korenčič   Group: iGEM07_Ljubljana   (2007-10-21)


CMV-myc-CXCR4-NUb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1178
    Illegal XhoI site found at 1874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1774
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is cloned into BBa_J52017 vector (pSBAK3 vector with eukaryotic terminator). c-myc tag is added.

Source

CXCR4 gene obtained from Origene, NUb gene obtained from Dualsystems. CMV originates from pcDNA3 by Invitrogen.

References